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Anna Gałązka

Anna Gałązka

Institute of Soil Science and Plant Cultivation State Research Institute, Poland

Title: The genetic and functional diversity of bacterial community in soils contaminated with crude oil

Biography

Biography: Anna Gałązka

Abstract

Soil contamination with crude oil, especially in the area of oil wells, is a serious environmental problem. Restoring the long – term pollution soil to its original state is very difficult. Under such conditions, the unique group of bacterial communities develops in the soil. This communities  are  adapted to the contamination conditions. Analysis of the structure and function of these microorganisms can be a source of valuable information. The aim of the study was to evaluate functional and structural diversity of bacterial communities in soils with long term impacts from crude oil. Samples were taken from four oil wells: one oldest and still working (W01, working from 1888) and three younger (W02, W03, W04, working from the beginning of 20th century) still working but with periodic breaks – these oil wells works only few months every years. Soil samples were collected at two distances: within a radius of 0.5 m of the oil wells (W01R, W02R, W03R, W04R) and within a radius of 3 m from the oil wells (W01, W02, W03, W04). The next generation sequencing technique (V3-V4 16S rRNA) was accompanied with the community level physiological profiling (CLPP) method in order to better understand knowledge of both genetic and functional structure of soils collected under several oil wells. The significant differences of bacterial community structure between soils were obtained. The soils taken directly from oil wells were characterized by different composition of bacteria. The highest activity of carbon utilization patterns were observed in soils taken directly from oil wells.