Patrizia Cesaro
Università Piemonte Orientale, Italy
Title: Exploring AM fungal community associated to Vitis vinifera cv. Pinot Nero treated with integrated pest managements using a metagenomics approach
Biography
Biography: Patrizia Cesaro
Abstract
Vitis vinifera (L.) is an economically important crop whose value depends largely on fruit quality. Most grapevine varieties are susceptible to diseases and different approaches can be chosen to control grapevine pests (conventional practice, integrate pest practice (IPM) and organic pest practice).
Among soil microorganisms, arbuscular mycorrhizal fungi (AMF) are able to establish symbiotic associations with vine roots. These fungi have beneficial effects on grapevine performance, including water use efficiency, vine biomass, replant success and soil nutrient uptake. Previous reports suggested a host specificity among grapevine and AM fungi and that soil characteristics and tillage may be important in determining this association.
In this study, we examined the AM fungal communities in the rhizospheric soil of V. vinifera cv Pinot Nero and bulk soil using 454 Roche sequencing technology.
The bulk soil and the rhizospheric soil of the grapevines were sampled before and after grape production. Genomic DNA was extracted and amplified, according to the methods optimized for 454 Roche pyrosequencing, by nested PCR using AMF specific primers of the large ribosomal subunit (LSU rDNA). Sequences were compared with both NCBI database and an AMF LSU rDNA reference database.
Our data showed different AMF communities in the rhizospheric and bulk soil of V. vinifera and the importance of the sampling time in regulating AMF biodiversity.