Institute of Soil Science and Plant Cultivation State Research Institute, Poland
Title: Determination of structural biodiversity and functional and metabolic profiles in soil under long-term maize monoculture
Biography: Anna Gałązka
Microbial diversity in soil may be limited under natural conditions by inappropriate environmental factors such as: limited food resources, environmental and physical factors, tillage system and interspecies interactions prevent the occurrence or maintenance of the species in the environment. The aim of this work was to determinate structural biodiversity and functional microorganisms in in soil under long-term maize monoculture. The reaction of maize cultivated in perennial monoculture for the direct sowing was investigated and compared to full tillage monoculture and crop rotation full tillage cultivation in the following phases: six leaves, twelve leaves, flowering phase, before harvest and after harvest. Three objects were included into this research: maize cropped continuously monoculture - zero tillage, maize monoculture cropped continuously – full tillage, crop rotation (spring barley, winter wheat, maize) – full tillage. The evaluation of the structural biodiversity of the soil was based on the next-generation sequencing (NGS). The functional and metabolic profiles in soil were determined by Biolog EcoPlate System. The research methods used in this subject have contributed to a better understanding of genetic diversity and composition of the population of microorganisms in the soil environment under the influence of the changes that have taken in soil under long-term maize monoculture.