Małgorzata Woźniak
Institute of Soil Science and Plant Cultivation State Research Institute, Poland
Title: Bacterial endophytes in crops – molecular identification and biodiversity
Biography
Biography: Małgorzata Woźniak
Abstract
Bacterial endophytes reside in a large number of plant species as part of their microbiome. Endophyte community structure exhibits a high diversity of species within a plant. This diversity is determined by abiotic and biotic factors such as soil conditions, biogeography, plant species, microbe– microbe interactions and plant–microbe interactions. The purpose of this study was to assess the degree of genomic diversity, relationship and molecular identification of bacteria isolated from the endosphere of maize, broad bean, wheat, rye, horsetail and burdock (root and stem). The tests were performed on 45 strains. The genetic identification of bacterial population was performed based on comparative sequence analysis of the 16S rDNA. On the basis of 16S ribosomal RNA sequences, all strains of bacteria were assigned to 10 genera: Rhizobium (19 isolates), Delftia (11 isolates), Agrobacterium (5 isolates), Stenotrophomonas (3 isolates), Brevundimonas (2 isolates), Novosphingobium (1 isolate), Variovorax (1 isolate), Collimonas (1 isolate), Achromobacter (1 isolate) and Comamonas (1 isolate). The assessment of the diversity of potential plant growth promoting bacteria, including 11 species Delftia sp, was conducted based on the BOX-PCR, ERIC-PCR and PCR-DGGE techniques. The genetic profile shows similarity among tested bacteria strains. The microorganism group was related to the type of the host plant.
This research was supported by the project nr 1.21 (2017 – 2019) the statutory activity of IUNG-PIB in Pulawy "Molecular and biochemical identification of endophytic bacteria and their use in plant growth promotion".