Microbial Interactions & Microbial Ecology

Biography

Biography: Małgorzata Woźniak

Abstract

The Paulownia spp. is a fast growing variety of deciduous tree that belongs to the Paulowniaceae family. These trees are used to produce biomass and reduce carbon dioxide concentrations in the atmosphere. Paulownia spp. leaves have a high content of protein, fats, sugar and nitrogen, phosphorus, potassium (NPP). The purpose of this study was to determine the functional and genetic diversity of the microbial of the Paulownia spp. Next Generation Sequencing (NGS) and Biolog EcoPlates were used to evaluate microbial diversity. The research materials were leaves of trees Paulownia spp. Plant samples were taken from Podkampinos (a plantation established on arable soil) and OtrÄ™busy (a plantation established on degraded soil). The samples were the clones of the same hybrid Paulownia elongata and Paulownia fortunei. Additional hybrid of Paulownia tomentosa and Paulownia fortunei was included. The functional analysis was carried out using statistical methods: cluster analysis by Ward’s method, taking into account Euclidean square of distance. Moreover, the Shannon-Wiener (H') biodiversity index, R Richness, E Evenness, and AWCD (average well color development) were calculated. The obtained sequences were subject to bioinformatics analyses, such as clustering and separation of operational taxonomic units (OTU). The samples from the plantation established on degraded soli revealed that the most dominant phylum was Bacteroidetes, whereas from the arable soil, Proteobacteria. Comparisons of carbon source utilization and the diversity indices showed differences in the microbial community of composition. Among the five carbon sources studied, carbohydrates were the most frequently used group of compounds.

This research funded by the NCN-Preludium project no 2016/23/N/NZ9/02157